Fig. 5.

Compound-1H induces the mitochondria-mediated intrinsic apoptosis via the regulation of apoptosis-related proteins in human glioblastoma cells. U87 and LN229 cells were treated with the compound-1H (0, 15, 30, 60 μmol/L) for 48 h. a Flow cytometry analysis of ∆Ψm by Rhodamine 123 staining. b The corresponding histogram shows the percentages of cells with high ∆Ψm (survival) and low ∆Ψm (apoptosis). Values represent the mean ± SD (n = 3). *P < 0.05; **P < 0.01; ***P < 0.001 compared with the control group. c After treatment with the compound-1H (0, 15, 30, 60 μmol/L) for 48 h, cell lysates of U87 and LN229 cells were prepared, and the expression of cleaved caspase-9, Bax, Bcl-2, cleaved caspase-3 and cleaved PARP were analyzed using western blot. β-Tubulin was used as a loading control