Figure 3. Effect of α-mangostin on migration of A549 cells.

The cells were treated with 5 and 10 µM α-mangostin for 24 h and were subjected to analyses for cell migration. (A) Phase contrast images of wound healing assay of A549 cells treated with α-mangostin. The cells were scratched and then cultured for 24 h. The scratched areas are shown with broken lines. (B) The ratio of the recovered wound area, A_r, by cell migration. The ratio was calculated from more than 10 images in each condition. α-Mangostin displayed an inhibitory effect on cell migration for A549 after 12 and 24 h. The Kruskal-Wallis H value and p-value of 12 h data are 8.36 and 0.015, respectively. The Kruskal-Wallis H value and p-value of 24 h data are 8.92 and 0.012, respectively. * p < 0.05 vs. A_r of control conditions (Steel pairwise comparison test).