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. Author manuscript; available in PMC: 2019 Jun 14.
Published in final edited form as: Chem Commun (Camb). 2018 Jun 14;54(49):6336–6339. doi: 10.1039/c8cc02003e

Figure 1. Peptide-based nanodiscs as a tool to unveil lipid-boundary regions of CPR.

Figure 1

4F-ER nanodiscs allow detergent-free incorporation and monomerization of CPR. (a) Schematic of the lipid-exchange experiment. CPR was reconstituted with a 2.5-fold excess of empty 4F peptide based nanodiscs containing ER membrane lippids and incubated overnight to enable lipid exchange between nanodiscs. (b) SEC profiles showing two partially overlapped peaks, representing the reconstituted and empty fractions; the insert depicts a TEM image of the reconstituted fraction.