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. Author manuscript; available in PMC: 2019 Jun 14.
Published in final edited form as: Chem Commun (Camb). 2018 Jun 14;54(49):6336–6339. doi: 10.1039/c8cc02003e

Figure 2. CPR lipid boundaries differ from membrane bulk composition and determine topological and structural fluctuations.

Figure 2

(a) 31P-NMR on detergent-treated nanodiscs was used to assess the phospholipid composition on the protein-containing fractions after overnight lipid exchange. (b) Percentage of lipids and cholesterol contents in empty, CPR, and cytb5 4F-ER nanodiscs after lipid exchange, as measured by 31P-NMR (phospholipids) and GC-MS (cholesterol). Data are expressed as average ± standard deviation (n = 3). * p<0.05, ** p<0.01, *** p<0.001. (c) Steady-state fluorescence emission of CPR in the 4F-nanodiscs (λex=450 nm). Φi is the relative quantum yield (to flavin) measured as described in the Experimental Section. (d–e) Steady-state fluorescence anisotropy of CPR and flFBD in solution and 4F-nanodiscs (λex=450 nm, λem = 525 nm). (f) Fluorescence lifetime decays measurements of CPR in solution or in 4F-nanodiscs nanodiscs (λex=450 nm, λem = 525 nm). IRF, instrument response function.