Table 1.
Polymerization rate (μm/min) | Depolymerization rate (μm/min) | Polymerization duration (s) | Depolymerization duration (s) | Catastrophe frequency (events/min) | Rescue frequency (events/min) | |
---|---|---|---|---|---|---|
Wild type | 1.59 (1.40–1.78) | 2.80 (2.52–3.07) | 40 (35–45) | 24 (21–27) | 1.00 (0.78–1.22) | 1.67 (1.31–2.02) |
tub2-430∆ | 1.57 (1.34–1.80) | 2.49 (2.19–2.78) | 44* (38–50) | 36* (32–41) | 0.69* (0.52–0.85) | 0.79* (0.53–1.06) |
tub2-438∆ | 1.61 (1.24–1.98) | 2.65 (2.30–3.00) | 40 (33–47) | 28 (24–32) | 0.94 (0.55–1.33) | 1.08* (0.69–1.48) |
tub2-polyQ | 1.31*(1.15–1.47) | 2.21* (2.01–2.41) | 43 (37–49) | 35* (31–39) | 0.82 (0.67–0.97) | 0.91* (0.58–1.23) |
Source data are available for this table.
Image series were collected at 3- to 4-s intervals for 10 min and microtubule lengths were measured at each time point. Values shown are the medians, with 95% CIs in parentheses, of pooled data from at least four separate experiments.
P < 0.05, compared with wild type, based on the Mann–Whitney U test. Number of aMTs measured for each genotype: wild type, n = 45; tub2-430∆, n = 51; tub2-438∆, n = 34; and tub2-polyQ, n = 65.