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. 2018 Apr 19;1(2):e201800054. doi: 10.26508/lsa.201800054

Table 1.

Dynamics of astral microtubules measured in yeast cells during preanaphase.

Polymerization rate (μm/min) Depolymerization rate (μm/min) Polymerization duration (s) Depolymerization duration (s) Catastrophe frequency (events/min) Rescue frequency (events/min)
Wild type 1.59 (1.40–1.78) 2.80 (2.52–3.07) 40 (35–45) 24 (21–27) 1.00 (0.78–1.22) 1.67 (1.31–2.02)
tub2-430∆ 1.57 (1.34–1.80) 2.49 (2.19–2.78) 44* (38–50) 36* (32–41) 0.69* (0.52–0.85) 0.79* (0.53–1.06)
tub2-438∆ 1.61 (1.24–1.98) 2.65 (2.30–3.00) 40 (33–47) 28 (24–32) 0.94 (0.55–1.33) 1.08* (0.69–1.48)
tub2-polyQ 1.31*(1.15–1.47) 2.21* (2.01–2.41) 43 (37–49) 35* (31–39) 0.82 (0.67–0.97) 0.91* (0.58–1.23)

Source data are available for this table.

Image series were collected at 3- to 4-s intervals for 10 min and microtubule lengths were measured at each time point. Values shown are the medians, with 95% CIs in parentheses, of pooled data from at least four separate experiments.

*

P < 0.05, compared with wild type, based on the Mann–Whitney U test. Number of aMTs measured for each genotype: wild type, n = 45; tub2-430∆, n = 51; tub2-438∆, n = 34; and tub2-polyQ, n = 65.

Source Data for Table 1LSA-2018-00054_SdataT1.xlsx (32.2KB, xlsx)