Fig. 4.

Positive allosteric activity of Cmpd-6 in agonist-induced β₂AR bimane signals. (A) The fluorescence emission spectrum of monobromobimane-labeled β₂AR in HDL particles. Data shown are representative of three independent experiments. (B) Bar graph summarizes the analyses, in which normalized peak fluorescence values are expressed relative to DMSO control (0.2%). Values indicate mean ± S.D. Statistical analysis for the results depicted as the bar graph was performed using one-way analysis of variance, repeated (related) measures with Tukey’s multiple comparison post-tests. Adjusted **P < 0.01; ***P < 0.001; ns, not significant.