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. Author manuscript; available in PMC: 2018 Jun 28.
Published in final edited form as: Anal Chem. 2017 Nov 21;90(1):144–165. doi: 10.1021/acs.analchem.7b04431

Figure 3. In vitro cross-linking strategies for protein complexes.

Figure 3

(A) 1- and 2-step in-solution in vitro cross-linking protocols for protein complexes. (B) 1- and 2-step on-bead in vitro cross-linking protocols for affinity purified protein complexes. 2-step protocols in (A) and (B) involve in vitro mild FA cross-linking prior to the second cross-linking step. (C) 2-step cross-linking protocol by coupling in vivo mild FA prefixing with on-bead in vitro cross-linking of affinity purified protein complexes.