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. Author manuscript; available in PMC: 2018 Jun 28.
Published in final edited form as: Anal Chem. 2017 Nov 21;90(1):144–165. doi: 10.1021/acs.analchem.7b04431

Figure 4. General scheme for integrative structural modeling.

Figure 4

Integrative structural determination of the protein complexes proceeds through four main stages: (1) data is first gathered from various structural elucidation methodologies; (2) subunits are represented from high-resolution structures while other forms of low-resolution structural data are translated into spatial restraints; (3) configurational sampling produces an ensemble of structures that satisfies translated restraints; and (4) the ensemble structure is analyzed and validated. Adapted from Wang, et al 98 (This research was originally published in J. Biol. Chem. Wang, X., Chemmama, I.E., Yu, C., Huszagh, A., Xu, Y., Viner, R., Block, S.A., Cimermancic, P., Rychnovsky, S.D., Ye, Y., Sali, A., Huang, L. The Proteasome-Interacting Ecm29 Protein Disassembles the 26S Proteasome in Response to Oxidative Stress. J Biol. Chem. 2017, 292(39):16310–16320. © the American Society for Biochemistry and Molecular Biology).