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. Author manuscript; available in PMC: 2018 Jun 28.
Published in final edited form as: Anal Chem. 2017 Nov 21;90(1):144–165. doi: 10.1021/acs.analchem.7b04431

Table 2.

Representative MS-cleavable Cross-linkers

Cleavable Bond Dissociation Type Reagent Workflow # Refs
C-S (Sulfoxide) CID graphic file with name nihms975790t1.jpg MSn (CID2, CID3)
MS2 (CID2, ETD2)
MS2 + MSn
[74, 95]
[101]
[102]
graphic file with name nihms975790t2.jpg MSn (CID2, CID3) [84,96]
graphic file with name nihms975790t3.jpg MSn (CID2, CID3) [85]
graphic file with name nihms975790t4.jpg MSn (CID2, CID3) [79, 93]
C-S (Sulfonium) CID graphic file with name nihms975790t5.jpg MSn (CID2, CID3) [70]
C-S (Cyanuric) CID graphic file with name nihms975790t6.jpg MS2 (CID2) [75]
D-P (Asp-Pro) CID graphic file with name nihms975790t7.jpg MS2 (IS1, CID2)
MSn (CID2, CID3)
[69, 86]
graphic file with name nihms975790t8.jpg MSn (CID2, CID3) [89, 103]
CID/ETD graphic file with name nihms975790t9.jpg MSn (CID2, CID3)
ETD2 validation
[88]
C-N (Rink) CID graphic file with name nihms975790t10.jpg MSn (CID2, CID3) [68, 87]
graphic file with name nihms975790t11.jpg MSn (CID2, CID3) [76]
C-N (Urea) CID graphic file with name nihms975790t12.jpg MS2 (CID) [73]
graphic file with name nihms975790t13.jpg MS2 (HCD) [90]
graphic file with name nihms975790t14.jpg MS2 (HCD) [91]
C-N (Quaternary diamine) CID graphic file with name nihms975790t15.jpg MSn (CID2, CID3) [92]
N=N CID (FRIPS) graphic file with name nihms975790t16.jpg MSn (HCD2, CID3) [112]
N-N (Hydrazone) ETD graphic file with name nihms975790t17.jpg MSn (ETD2, CID3) [71]
C-N ETD graphic file with name nihms975790t18.jpg MS2 (ETD2) [72]