Figure 4.

Lechevalieria aerocolonigenes K10-0216 and the mangromicins discovered from the culture broth. (a) Scanning electron micrograph of a clump of interwoven hyphae of L. aerocolonigenes K10-0216 grown on inorganic salt–starch agar at 27 °C for four weeks; (b) Structure of mangromicin A–I; (c) Productivity of mangromicin A. Black circle: Basic medium; soluble starch 2.0(%), defatted wheat germ 1.0, glycerol 0.5, dry yeast 0.3, CaCO₃ 0.5, meat extract 0.5. Open circle: Improved medium; soluble starch 5.0(%), defatted wheat germ 1.0, glycerol 0.5, dry yeast 1.0, CaCO₃ 0.5, meat extract 0.0. (d) HPLC analysis of the mangromicins. Chromatographic separation was undertaken using a MonoBis (3.2 × 150 mm, Kyoto Monotech Co., Ltd., Kyoto, Japan) at 40 °C. With regard to gradient elution, solvent A was water with 0.1% formic acid, and solvent B was methanol with 0.1% formic acid. The gradient elution was 0–10 min and 5–100% B. The flow rate was 0.5 mL/min, the injection volume was 5 μL, and detection occurred at 254 nm using a photodiode array detector.