Table 4.
Oligonucleotide primers employed for DNA sequence analysis and plasmid constructions.
| Name | λ Map Position | Sequence (5′ to 3′) a |
|---|---|---|
| L-37904+18 | 37904–37922 | GCTGCTCTTGTGTTAATGG |
| L-MH29 | 37905–37922 | CGTCCTCAAGCTGCTCTTGTGTTAATGG |
| L20 | 39465–39484 | ACTCCGCGATAAGTGGACCC |
| L-22 | 38517–38534 | TGCTGCTTGCTGTTCTTG |
| L-PG30 | 38530–38547 | TTGGAACTGAGAAGACAG |
| L-PG1 | 38784–38801 | AAATATGCTGCTTGAGGC |
| L-38985p20 | 38985–39005 | GCAGCAAGGCGGCATGTTTGG |
| L-MH32 | 39531–39550 | CACAGATCTATAGCAAACCAAAACTCGACCTGA |
| L-18 | 39980–39996 | TTGCCGGAAGCGAGGCC |
| L-21 | 40360–40377 | CGCAACAGTAACCAGCAT |
| R-PG2 | 40747–40764 | GGTTGCGTTCCTGAATGG |
| L-Bam-O | 38686–38718 | ATATGGATCCATGACAAATACAGCAAAAATACTCAACTTCGGC |
| L-Bam-P | 39582–39606 | ATATGGATCCATGAAAAACATCGCCGCACAGATGG |
| L-Bam-OOP#1 | 38559–38580 | ATATGGATCCTGGCTCGATTGGCGCGACAAGT |
| L-Bam-OOP#2 | 38546–38577 | ATATGGATCCGTTGACGACGACATGGCTCGAT |
| L-Bam-O | 38686–38718 | ATATGGATCCATGACAAATACAGCAAAAATACTCAACTTCGGC |
| L-Bam-P | 39582–39606 | ATATGGATCCATGAAAAACATCGCCGCACAGATGG |
| R-40769m22 | 40747–40769 | GCTGCGGTTGCGTTCCTGAATGG |
| R-MH33 | 40315–40295 | GCGACGTCCCCAGGTAATGAATAATTGC |
| R-17 | 40018–40002 | TAAGACTCCGCATCCGG |
| R-MH25 | 39626–39609 | CTGCTCACGGTCAAAGTT |
| R-39280m21 | 39259–39280 | CTGCGGCGGTCAGGTCTTCTGC |
| R9+1 | 39191–39175 | TGGTCAGAGGATTCGCC |
| R-PG6 | 38569–38552 | CAATCGAGCCATGTCGTC |
| R-1536-19 | pcIpR-()-timm | GAAGACAGTCATAAGTGCGG |
| R-ClaI-P | 40280–40259 | ATATATCGATTATACACTTGCTCCTTTCAGTCCG |
| R-ClaI-O | 39582–39559 | ATATATCGATTATAGATCCACCCCGTAAATCCAGTC |
| R-ClaI-36P | 39687–39662 | ATATATCGATTACCTGCTGTACCTGCGGCTTTTCGTCG |
| R-ClaI-63P | 39768–39746 | ATATATCGATTACTTCGTTCTGGTCACGGTTAGCC |
| R-AscI-O | 39582–39559 | ATATGGCGCGCCGCTGCCGCCTAGATCCACCCCGTAAATCCAGTC |
a The portion of primer sequences shown in smaller font size contain restrictions sites used for cloning into plasmids and are not included within λ map sequence shown.