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. 2018 Apr 26;8(2):21. doi: 10.3390/biom8020021

Figure 2.

Figure 2

Evaluation of the miRNeasy (Qiagen) and miRCURY (Exiqon) extraction kits. (A) Eight healthy blood donors were used. RNA was extracted from 200 µL serum using the miRNeasy RNA isolation kit (Qiagen) and miRCURY RNA isolation kit Biofuids (Exiqon) following the manufacturer’s instructions, and eluted in 14 µL and 50 µL of RNase free water respectively. TaqMan microRNA quantitative reverse transcription polymerase chain reaction (qRT-PCR) (Applied Biosystems, Waltham, MA, USA) was carried out against miR-423-3p, a normalizer recommended by Exiqon. Cq values for miR-423-3p obtained from RNA extracted using the miRNeasy RNA isolation kit (Red) verses Cq values obtained from RNA extracted using the miRCURY RNA isolation kit Biofluids (Green) are displayed. (B) Eighteen healthy blood donors were used. RNA was extracted from 200 µL serum using the miRCURY RNA isolation kit Biofluids (Exiqon). TaqMan microRNA qRT-PCR and miRCURY™ LNA™ Universal RT microRNA polymerase chain reaction (PCR) (Exiqon) was carried out against miR-423-3p. Cq values for miR-423-3p obtained using the TaqMan chemistry are displayed in blue and the Cq values for miR-423-3p obtained using the SYBR-Green based (Exiqon) chemistry are displayed in yellow (see also Supplementary data). *** p < 0.001.