Figure 4.

S-layer-coated liposomes and emulsomes. TEM images of emulsomes coated with the S-layer protein SbsB from Geobacillus stearothermophilus PV72/p2 (A) wildtype SbsB and (B) recombinant SbsB. The bars correspond to 100 nm. Adopted from [191], copyright (2013) with permission from Wiley-VCH. (C) Schematic drawing of (1) an S-layer coated emulsomes (left) and Iiposome (right) with entrapped functional molecules and (2) functionalized by reconstituted integral proteins. Note, S-layer coated emulsomes can only transport hydrophobic molecules but with a much higher transport capacity. S-layer coated emulsomes and liposomes can be used as immobilization matrix for functional molecules (e.g., human IgG) either by direct binding (3) or by immobilization via the Fc-specific ligand protein A (4), or biotinylated ligands can be bound to the S-layer coated liposome or emulsomes via the biotin—avidin system (5). Alternatively, emulsomes or liposomes can be coated with genetically modified S-layer subunits incorporating functional domains (6). Modified after [61], copyright (2002) with permission from Wiley-VCH.