Figure 6.

Recognition of TORC2-specific substrate Ypk2 by chimera 273. (A) Autoradiograph of triplicates of in vitro kinase reactions using immuno-purified complexes containing indicated chimeras and recombinant GST-Ypk2. (B) Western blot of loading controls demonstrating that essentially equivalent amount of TOR chimeras were present in the immunoprecipitated complexes (bound (B) fractions) used for kinase reactions. (C) Quantification of data in (A), where total phosphorimager units are indicated. Data are presented as means +/− standard deviation (SD) of the triplicate samples shown in (A) and were normalized for levels of Tor protein present.