Table 1.
Growth properties of TOR1–TOR2 chimeras 1.
| Plasmid | WT 30 °C + Rap | tor1Δ 30 °C + Rap | tor2Δ 30 °C | tor2Δ 37 °C | tor2Δ 37 °C + Sorbitol | tor2Δ 37 °C + YPD | tor2Δ 37 °C + YPD +Sorbitol |
|---|---|---|---|---|---|---|---|
| pRS315 | 0 | 0 | 0 | ND | ND | ND | ND |
| pPL130 | +++++ | +++++ | 0 | ND | ND | ND | ND |
| pPL132 2 | 0 | 0 | 0 | ND | ND | ND | ND |
| pPL089 2 | 0 | 0 | +++++ | +++++ | +++++ | +++++ | +++++ |
| pPL268 | +++++ | +++++ | +++++ | + | ++ | + | +++++ |
| pPL172 2 | ND | ND | +++++ | +++++ | +++++ | +++++ | +++++ |
| pPL271 | +++++ | +++++ | +++++ | 0 | ++ | ++ | +++++ |
| pPL273 | +++++ | +++++ | +++++ | + | ++ | ++++ | +++++ |
| pPL175 | +++++ | +++++ | +++++ | ++ | +++ | ++++ | +++++ |
| pPL176 | +++++ | +++++ | ++++ | + | + | ++ | +++++ |
| pPL212 | ++++ | +++++ | +++++ | + | ++ | ++++ | +++++ |
| pPL180 | +++++ | +++++ | ++++ | 0 | 0 | 0 | + |
| pPL184 | +++ | ++++ | ++++ | 0 | <+ | <+ | ++++ |
| pPL185 | +++++ | +++++ | +++ | 0 | 0 | 0 | <+ |
| pPL209 | +++++ | +++++ | +++ | 0 | 0 | 0 | ++++ |
| pPL214 | ++++ | ++++ | +++++ | 0 | + | 0 | ++++ |
| pPL321 2 | ND | ND | +++++ | +++++ | +++++ | +++++ | +++++ |
| pPL333 2 | ND | ND | +++++ | +++ | ++++ | ++++ | +++++ |
| pPL270 | +++++ | +++++ | 0 | ND | ND | ND | ND |
| pPL173 | +++++ | +++++ | 0 | ND | ND | ND | ND |
| pPL177 | ++++ | +++ | 0 | ND | ND | ND | ND |
| pPL183 | +++ | ++++ | 0 | ND | ND | ND | ND |
| pPL182 | ++++ | ++++ | 0 | ND | ND | ND | ND |
| pPL186 | +++++ | +++++ | 0 | ND | ND | ND | ND |
1 Cell growth was determined by relative colony size following incubation for 2–4 days on solid medium: +++++ corresponds to wild type (WT) growth and 0 corresponds to no growth. ND = Not Determined. + Rap = growth in the presence of 0.2 µg/mL rapamycin. The WT control strain refers to strain W303a and tor1Δ and tor2Δ strains are derived from this strain. Plasmid pRS315 is an empty control vector; pPL130 carries the TOR1-1 gene; pPL132 carries the WT TOR1 gene; pPL089 carries the wild type TOR2 gene. Other plasmids are described in Figure 1 and Table 4. 2 Plasmid does not carry the TOR1-1 allele. Therefore, its ability to confer TORC1 activity was tested by the ability to confer WT growth within a W303a tor1Δ strain at 37 °C.