Figure 1.

Principle of the experimental design and data analysis. (A) Antibody patterns are used to enrich and immobilize CD59-monomeric green fluorescent protein (mGFP) at specific sites in the plasma membrane of living T24 cells. Cells are interfaced with microstructured surfaces containing different densities of antibody, so that different surface densities of immobilized CD59-mGFP can be adjusted; (B) Typical region of interest in a micropatterned cell showing enrichment of CD59-mGFP in “ON” areas and depletion in “OFF” areas. Scale bar is 3 µm; (C) Sketch depicting individual immobilized CD59-mGFP molecules (dots) in “ON” areas. Trajectories of fluorescently labeled lipids (shown in red) are recorded and separated into the categories “ON” and “OFF” according to the CD59-mGFP patterns recorded in the GFP color channel.