Figure 3.

Dioleoyl-phosphatidylethanolamine(DOPE)-PEG-KK114 diffusion decreases with increasing CD59-mGFP density; the relative mobility of both lipids is independent of cell cholesterol levels. (A) Mean square displacements of DOPE-PEG-KK114 “ON” (orange) and “OFF” (black) CD59-mGFPI-enriched areas are plotted as a function of t_lag for one representative cell. Diffusion coefficients were determined by fitting the function msd = 4Dt_lag + 4σ_xy²; (B) Relative diffusion coefficients D_ON/D_OFF were determined for individual cells (N = 28), plotted as a function of CD59-mGFP density, ρ_CD59-mGFP, and fitted with Equation (2) (orange line). The gray circle indicates the cell shown in (A). Relative diffusion coefficients D_ON/D_OFF are plotted as a function of CD59-mGFP density (open diamonds) and after treatment with 1 U/mL cholesterol oxidase (closed diamonds) for (C) DOPE-PEG-KK114 (N = 19) and (D) Chol-PEG-KK114 (N = 17). Data from cholesterol-depleted cells were fitted with Equation (2) (orange line).