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. 2018 May 28;7(2):33. doi: 10.3390/biology7020033

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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DdRabS overexpressing cells have decreased chemotactic activity in response to cAMP, and cell cohesion is decreased in DdRabS overexpressing cells. (A) Micrograph of chemotactically moving cells. After 4 h, the Dictyostelium cells moved beyond one field of view on the agarose in a 60 × 15 mm Petri dish. The amoeboid control cells, WT AX4, clearly showed trails toward the source of cAMP. Cells overexpressing RabS showed a decreased response to cAMP (arrows) while RabS(DN) cells moved towards the source of cAMP at a similar rate as WT AX4 cells. Cell cohesion was evaluated by monitoring the reassociation of cells over time without (B) or with (C) the addition of 10 mM EDTA. Cells that were not in aggregates at time zero and the percentage of cell reassociation was calculated by scoring non-aggregated cells with an hemocytometer (percentage aggregation = (total number of non-aggregating cells)/total number of cells). RabS overexpressing cells have decreased cohesion compared to the WT AX4 cells. Data represent the mean of three independent experiments. Data was run through a student’s t-test with p ≤ 0.05.