Figure 8.

Cell death quantification using flow cytometry analysis; RabS overexpressing cells have decreased metabolic activity and regrowth ability as compared to controls. (A–D) Cell death quantification using flow cytometry analysis. (A) Cells were stained with 1 µg/mL Propidium Iodide, and fluorescent PI-positive cells were quantified using flow cytometry. Dot plot data with side scatter and forward scatter showed dead cells distinct from living cells. RabS over-expressing cells show an increased level of induced cellular death as compared to the WT AX4 cells. (A) Histogram of the dot plot data. (B–D) Graphical representation with standard error. (B) Quantification for DIF-1 treated cells undergoing apoptosis. RabS overexpressing cells show an increase in induced cell death. (C) Metabolic activity showed as a decrease in the luminescent signal from the RabS overexpressing as compared to the AX4 cell line. (D) Regrowth ratios of the mutant cells after 72 h of treatment with DIF-1. RabS overexpressing cell lines showed a decreased survival rate compared to the WT AX4 and pDneo2a-GFP cell lines. Data represent the mean of three independent experiments. Data was run through a 2-way ANOVA to test for significance at p < 0.05.