Table 1.
Moonmilk strains isolated using the RC method and according to selective media.
| MMun Strains | Family | Closest Match (BLAST) | 16S rRNA Ident. %; gaps | Isolation Medium | Phylotype | OTU |
|---|---|---|---|---|---|---|
| 129 ♣ | Nocardiaceae | Nocardia sp. strain 2M-SSA4 | 99.40; 1 | S-SN + Fe + DFB | - | 25 |
| 130 ♣ | Paenibacillaceae | Paenibacillus sp. DSL09-3 | 98.96; 1 | S-SN + Fe + DFB | - | na |
| 131 ♣ | Streptomycetaceae | Streptomyces sp. 2323.1 | 99.61; 0 | S-SN + Fe + DFB | XXXII | 15 |
| 133 ♣ | Nocardiaceae | Nocardia sp. strain 2M-SSA4 | 99.14; 3 | S-SN + Fe + DFB | - | 25 |
| 135 ♣ | Streptomycetaceae | Streptomyces sp. MM63 | 100; 0 | SN + Fe | IV * | 21 |
| 136 ♣ | Nocardiaceae | Nocardia soli strain DSM 44488 | 100; 0 | SN + Fe | - | 25 |
| 137 ♣ | Streptomycetaceae | Streptomyces sp. MM82 | 99.08; 2 | SN + DFB | XXXIII | 30 |
| 138 ♣ | Streptomycetaceae | Streptomyces sp. MM63 | 100; 0 | SN | IV * | 21 |
| 139 ♣ | Streptomycetaceae | Streptomyces sp. MM63 | 100; 0 | SN | IV * | 21 |
| 140 ♣ | Nocardiaceae | Nocardia sp. strain 2M-SSA4 | 99.54; 1 | SN | - | 25 |
| 141 ♣ | Streptomycetaceae | Streptomyces sp. MM82 | 99.15; 2 | SN | XXXIV | 30 |
| 142 ♣ | Rhodobacteraceae | Uncult. Paracoccus sp. clone 54 | 99.93; 0 | SN + catalase | - | na |
| 143 ♣ | Streptomycetaceae | Streptomyces lunaelactis strain MM126 | 100; 0 | SN + catalase | X * | 21 |
| 144 ♣ | Nocardiaceae | Nocardia soli strain Y48 | 99.34; 0 | SN + catalase | - | 25 |
| 145 ♣ | Nocardiaceae | Rhodococcus sp. MTM3W5.2 | 99.80; 1 | SN + catalase | - | 1 |
| 146 ♣ | Streptomycetaceae | Streptomyces sp. MM44 | 100; 0 | S-SN + DFB | XXIX * | 21 |
| 147 ♣ | Streptomycetaceae | Streptomyces xiamenensis strain 318 | 98.69; 8 | S-SN + DFB | XXXV | 39 |
| 148 ♣ | Streptomycetaceae | Streptomyces xiamenensis strain 318 | 98.69; 8 | S-SN + DFB | XXXV | 39 |
| 149 | Micrococcaceae | Micrococcus sp. 3455 | 100; 0 | SN | - | 23 |
| 150 ♣ | Streptomycetaceae | Streptomyces sp. MM63 | 100; 0 | SN | IV * | 21 |
| 151 ♣ | Streptomycetaceae | Streptomyces sp. MM110 | 100; 0 | SN | XXI * | 21 |
| 152 | Streptomycetaceae | Streptomyces lunaelactis strain MM126 | 100; 0 | SN + Fe | X * | 21 |
| 153 | Streptomycetaceae | Streptomyces sp. MM18 | 99.93; 1 | SN + DFB | I * (lost) | 198 |
| 154 ♣ | Streptomycetaceae | Streptomyces sp. ND04-1H | 99.93; 0 | SN | XXXVI | 21 |
| 155 | Nocardiaceae | Rhodococcus sp. strain MAK1 | 99.86; 2 | SN | - | 421 |
| 156 | Streptomycetaceae | Streptomyces sp. 1C-HV8 | 99.86; 2 | S-SN + Fe + DFB | XXXVII | 132 |
| 157 ♣ | Streptomycetaceae | Streptomyces sp. 2M-TWYE1 | 99.47; 0 | SN + catalase | XXXVIII | 61 |
| 158 | Streptomycetaceae | Streptomyces sp. SpC090624KE_06 | 99.51; 0 | S-SN + Fe + DFB | XXXIX | 21 |
| 159 | Microbacteriaceae | Agromyces sp. strain 4K403B | 97.88; 14 | S-SN + DFB | - | 49 |
| 160 | Micrococcaceae | Kocuria rhizophila strain R-42745 | 99.93; 0 | S-SN | - | 159 |
| 161 | Streptomycetaceae | Streptomyces sp. MM18 | 100; 0 | S-SN + Fe + DFB | I * | 198 |
| 162 | Streptomycetaceae | Streptomyces sp. MM63 | 99.93; 1 | S-SN + Fe + DFB | IV * | 21 |
| 163 | Streptomycetaceae | Streptomyces sp. MM63 | 100; 0 | S-SN | IV * | 21 |
| 164 | Streptomycetaceae | Streptomyces sp. MM63 | 100; 0 | S-SN | IV * | 21 |
| 166 | Streptomycetaceae | Streptomyces sp. strain A301 | 99.23; 3 | S-ISP5 + Fe + DFB | XXXIV | 30 |
| 167 | Microbacteriaceae | Agromyces cerinus strain DSM 8595 | 99.58; 0 | ISP5 + catalase | - | 113 |
| 168 | Streptomycetaceae | Streptomyces sp. 28a-5-1 | 98.60; 8 | ISP5 + catalase | XXXV | 39 |
| 170 | Streptomycetaceae | Streptomyces sp. 2C-SSA16-1 | 99.64; 0 | S-ISP5 + Fe + DFB | XL | 61 |
| 171 | Pseudonocardiaceae | Amycolatopsis sp. CA11 | 98.25; 16 | S-ISP5 + Fe + DFB | - | 282 |
| 172 | Micromonosporaceae | Micromonospora maoerensis strain NEAU-MES19 | 99.93; 0 | S-ISP5 + Fe + DFB | - | 64 |
| 174 | Streptomycetaceae | Streptomyces sp. MM110 | 100; 0 | ISP5 + catalase | XXI * | 21 |
| 176 | Streptomycetaceae | Streptomyces sp. 28a-5-1 | 98.67; 7 | S-ISP5 | XXXV | 39 |
Numbers in the phylotype column have been deduced from the phylogenetic tree presented in Figure 4 and based on our previous screening [11]. The last column indicates to which OTU identified in our metagenetic study [13] the MMun strains are affiliated (according to the topology and patristic distances of the tree in Supplementary Figures S1 and S2). Abbreviations: S- before SN and ISP5, indicates that media were prepared by autoclaving of agar and phosphate separate from other media components; DFB, desferrioxamine B; na, not Actinobacteria; *, phylotypes described in our previous study [12]; lost, culture from mycelium stocks of MMun153 are no longer possible, and this strain is now considered as lost; and ♣, MMun isolates for which the genome has been sequenced.