Figure 3.

Quantitation of HHO1p in the cell. Western analysis was performed comparing known amounts of recombinant GST–HHO1p–2HA (58 kDa) with unknown quantities of HHO1p–2HA (30 kDa). Lanes 1–7 contain 37.5, 18.7, 9.4, 4.7, 2.3, 1.2 and 0.6 ng GST–HHO1p–2HA, respectively. Lanes 8–14 contain whole cell lysate from 13.2 × 10⁷, 6.6 × 10⁷, 3.3 × 10⁷, 1.6 × 10⁷, 0.8 × 10⁷ and 0.4 × 10⁷ cells, respectively.