Fig 2. MALT1 autoprocesses itself in vitro.

His-tagged BCL10, His-tagged MALT1 or MALT1_C464A mutant protein was expressed in E. coli and purified respectively. 1 μg purified full length MALT1 or catalytic-inactive mutant MALT1_C464A alone or in combination with 50 ng purified BCL10 proteins were incubated for 4 hr at 30°C in 50 mM MES (pH 6.8), 150 mMNaCl, 10% sucrose, 0.1% CHAPS, 10 mM DTT, and 1 M ammonium citrate. The reaction mixtures were resolved by SDS/PAGE, followed by Western blot analysis using anti-MALT1 or anti-BCL10 antibody. Data are representative of three separate experiments.