Fig 3. MiR-210 inhibits cell proliferation in CoCl2-induced pseudohypoxia.
(A) The expression level of miR-210 was detected in the stably miR-210 overexpressing KKU-213 and (B) in the stable knockdown miR-210 sponge KKU-100 cells compared to control cells. (C) A cell proliferation assay was done in stable KKU-213 cell cultured in normoxia (N) and pseudohypoxia (H). (D) A cell proliferation assay was done in stable KKU-100 cells cultured in normoxia and pseudohypoxia. The results for absorbance values at 540 nm are presented as mean ± SD. (E, F) A clonogenic assay was performed in stably overexpressing KKU-213 cells and their control cultured in normoxia (upper panel) and pseudohypoxia (lower panel) for 10 days. (G, H) A clonogenic assay was performed in stable knockdown KKU-100 cells and their control cultured in normoxia (upper panel) and pseudohypoxia (lower panel) for 14 days. *P < 0.05. **P < 0.01. ***P < 0.001.