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. 2018 Jun 28;13(6):e0199827. doi: 10.1371/journal.pone.0199827

Fig 6. MiR-210 mediates HIF-1α activity though targeting HIF-3α.

Fig 6

(A) HRE (hypoxia responsive element)-reporter assay in KKU-213 cells KKU-213 cells transiently transfected with 50 nM of HIF-3α siRNA compared with control cells (mock). (B) The in silico prediction using TargetScan database demonstrated the miR-210 binding site on 3’UTR of HIF-3α in both wild-type and mutant forms. (C) The 293T cells were transfected with Luc-HIF-3α-3’UTR wild-type or mutant reporter vector as well as miR-210 expression and control vectors. After 72 h transfection, the cells were lysed and the luciferase activity was detected. Data are presented as the luciferase activity normalized with the luciferase activity of Renilla. (D, E) Protein level of HIF-3α in the miR-210 overexpressing cells cultured in normoxia (N) and pseudohypoxia (H). The data present the mean ± SD of three-independent experiments. **P < 0.01. ***P < 0.001.