Fig 2. Vector competence in Aedes mosquitoes.

Dissemination efficiency (DE; proportion of mosquito with infectious virus in the head) was determined by testing mosquito heads with a TCID₅₀ assay (Panel A). The intensity of viral dissemination was assessed by comparing amounts of infectious particles in collected heads (Panel B; only positive heads were taken into account). Transmission efficiency (TE; proportion of mosquitoes with infectious virus in the saliva) was determined by testing mosquito saliva with a TCID₅₀ assay (Panel C). The intensity of viral transmission was assessed by comparing amounts of infectious particles in collected saliva (Panel D; only positive saliva were taken into account). In panels B and D, the average values are shown and error bars represent standard deviation. IC and ISA mean group of mosquitoes infected by IC_virus and ISA_virus respectively. “dpi” means days post-infection.