Table 1. Subtstitutions generated using the ISA method and detected in Aedes mosquitoes infected by the ISA_virus.
| Nucleotide position | Original substitution frequency* | Region | nucleotide change | aa change | Number of mosquitoes with the substitution detected on viral genome | ||
|---|---|---|---|---|---|---|---|
| A. aegypti 6 dpi (n = 8) | A. aegypti 9 dpi (n = 6) | A. albopictus 6 dpi (n = 5) | |||||
| 502 | 0,21 | nsP1 | A→G | - | 1 | 0 | 1 |
| 710 | 0,03 | nsP1 | T→C | - | 0 | 0 | 0 |
| 881 | 0,03 | nsP1 | A→C | K→Q | 0 | 0 | 0 |
| 1105 | 0,03 | nsP1 | C→A | - | 0 | 0 | 0 |
| 1590 | 0,22 | nsP1 | T→G | L→R | 1 | 0 | 1 |
| 2458 | 0,28 | nsP2 | T→C | - | 4 | 0 | 2 |
| 6923 | 0,23 | nsP4 | A→G | T→A | 3 | 2 | 4 |
| 7628 | 0,28 | C | C→T | P→L | 4 | 0 | 2 |
| 8612 | 0,02 | E2 | A→G | E→G | 0 | 0 | 0 |
| 8766 | 0,15 | E2 | A→G | - | 4 | 0 | 2 |
| 9100 | 0,1 | E2 | A→G | N→D | 0 | 3 | 1 |
| 9690 | 0,02 | E2 | T→C | - | 0 | 0 | 0 |
| 11644 | 0,1 | 3'UTR | A→G | - | 0 | 3 | 1 |
List of the substitutions detected in virus stock (ISA_virus) used to infect Aedes mosquitoes. None of these mutations were detected in mosquitoes infected by the IC_virus. “dpi” means days post-infection.
* frequency found in virus stock.