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. 2018 Jun 18;14(6):e1007143. doi: 10.1371/journal.ppat.1007143

Fig 5. SIV gp120/140 reactivity at week 20 post rRRV-SIVcmv-nfl inoculation.

Fig 5

A) Sera obtained at week 20 following rRRV-SIVcmv-nfl inoculation were serially diluted and tested for the reactivity to gp120 via an ELISA using recombinant SIVmac239 gp120 to coat the ELISA plates. Subsequently, reactive antibodies were detected with a horseradish-peroxidase-conjugated anti-rhesus IgG antibody. Furthermore, sera of two SIVmac239Δnef-infected animals (week 18 post infection) and sera obtained from two monkeys that had received rRRV expressing a codon-modified version of SIVmac239 gp160 (week 19 post inoculation) were tested [24]. B) Similarly, the monkey sera were serially diluted and tested for reactivity to gp140 by ELISA, using recombinant SIVmac239 gp140 to coat the ELISA plates. Reactive antibodies were detected with a horseradish-peroxidase-conjugated anti-rhesus IgG antibody.