Figure 1.

Workflow summarizing the main steps required for the characterization of genes important for different stages of the establishment of a functional symbiosis using functional genomics approaches. First, the investigated growth conditions are shown (first chevron), then the functional genomics technologies (second chevron) and their integrative data analyses (third chevron) used to prioritize candidate genes (fourth chevron). Finally, genes important for the different steps of symbiosis are identified and validated (fifth chevron). MM: minimal medium; 2D-GE: 2-dimensional gel electrophoresis; RNA-seq: RNA-sequencing.