Relative adult bees infestations in three time periods, determined using the sugar shake method. Adult bee infestations before the application of four acaricides, Apiguard®, Apistan®, Apivar®, and HopGuard® (period before treatments from 31 May–19 July, shown as July in figure) and after treatments (19 July–18 August, shown as August and September in figure, respectively). Denote that colonies of the control group received the acaricide treatment on 18 August and therefore a high Varroa infestation was recorded, as August testing, just before CheckMite® treatment. CheckMite® was applied as a final treatment to kill any remaining Varroa mites in all of the colonies. Varroa infestation levels (% ± SE) were calculated based on the relative number of mites remaining on the adult bees in each colony. The horizontal brackets indicate the mean comparison between the control and Apiguard® and Apistan® as a single treatment; * p < 0.05; ** p < 0.005.