Table 2.
Identification and application of BBB shuttle peptides identified from phage display library
| Peptide | Sequence (phage displayed library) | BBB model (in vitro and in vivo) | Transport mechanism | Sanger/(NGS) | Advanced application– (Nano formulations) | References |
|---|---|---|---|---|---|---|
| SGV |
SGVYKVAYDWQH (Ph.D.™-12 Phage Display) |
Co-cultured Endothelial cells and pericytes | N.A. | Sanger (31 clones) | N.D. | (Díaz-Perlas et al. 2016; Sánchez-Navarro et al. 2017) |
| Peptide-22 |
Ac-[cMPRLRGC]c-NH2 (15-mer cyclic phage library) |
CHO cells (h LDLR +) | LDLR-RMT | N.A. | PEG-PLA-peptide22-PTX; PEG-LIP-Peptide22-RGD-DOX. | (Malcor et al. 2012; Zhang et al. 2013a; Chen et al. 2017) |
| B6, B8 | B6: GHKAKGPRK B8: KWKTPKVRV (custom phage-display 9 mer library) |
Purified extracellular domain of hTfR | TfR-RMT | Sanger (43 clones) | N.D. | (Xia et al. 2000) |
| T7 or HAI |
HAIYPRH (Ph.D.™-7 Phage display Peptide Library) |
CEF (hTfR +) | TfR-RMT | Sanger (9 clones from 10th round) | PEG-LIP-T7-TAT-DOX; PEG-LIP-T7-ZL006; PEG-PLGA-T7-BCNU; PEG-PLGA-T7-PTX-BCNU; PEG-LIP-T7-DOX-VCR. | Lee et al. 2001; Zong et al. 2014; Wang et al. 2015b; Bi et al. 2016; Cui et al. 2016; Zhang et al. 2017) |
| THR |
THRPPMWSPVWP (Ph.D.™-12 Phage Display) |
CEF (hTfR +) | TfR-RMT | Sanger (9 clones from 7th round) | 68Ga-THR fallypride-THR | (Lee et al. 2001; Wängler et al. 2011) |
| G23 |
HLNILSTLWKYRC (Ph.D.™-12 Phage Display) |
Immobilized ganglioside GM1 | GM1/GT1b-RMT | Sanger (N.A.) | G23fluor polymersomes | (Georgieva et al. 2012) |
| PepC7 |
CTSTSAPYC (Ph.D.™-C7C Phage Display) |
ICR mice | N.D. | Sanger (12 clones) | N.D. | (Li et al. 2012) |
| CRT |
CRTIGPSVC (Ph.D.™-C7C Phage Display) |
BALB/c mice (i.v. tail vein) | TfR-RMT | Sanger | PEG-PLGA-CRT-PTX | (Staquicini et al. 2011; Kang et al. 2015) |
| GLA GYR |
GLAHSFSDFARDFVA GYRPVHNIRGHWAPG (Filamentous f3-15mer) |
C57Bl/6 mice (In situ Perfusion) | N.D. | Sanger (17 clones) | P3-GLA-LIP | (Van Rooy et al. 2010, 2012) |
| TGN |
TGNYKALHPHNG
(Ph.D.™-12 Phage Display) |
ICR mice | N.D. | Sanger (20 clones) | PEG-PLA-TGN; PEG-PLGA-TGN; PEG-PLA-LIP-TGN-PTX | (Li et al. 2011, 2013, Gao et al. 2012, 2014, Zhang et al. 2013b, 2014; Ma et al. 2014) |
| c-SxTSSTx-c |
C-SYTSSTM-C (Ph.D.™-C7C PhageP |
Sprague-Dawley (SD) rats | N.D. | Sanger (500 clones) | N.D | (Smith et al. 2012) |
| CAGALCY |
CAGALCY (T7-select-415-CX10 C) |
6 Balb/C mice | N.D. | Sanger (200 clones) | CAGALCY-AgNP | (Fan et al. 2007; Toome et al. 2017) |
| #2077 |
RLSSVDSDLSGC (T7-select 10-3b-12 mer) |
Wistar rats | N.D. | NGS | N.D. | (Urich et al. 2015) |