Figure 4.

Treating zebrafish embryos with Anti-U94 morpholino causes changes in exon retention of cardiac regulatory genes. 13 of 39 members of the WNT family have changes in exon retention after treatment with antisense morpholino (assessed by RNA-Seq and qRT-PCR, Values shown are from qRT-PCR data). Data are fold change of exons that represents regions of variability within the alternatively spliced gene. (Each gene analyzed by qRT-PCR was done on 3 separate embryo samples with 3 technical replicates). * significant p < 0.05.