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. Author manuscript; available in PMC: 2018 Jun 28.
Published in final edited form as: Bioconjug Chem. 2017 Oct 27;28(11):2874–2886. doi: 10.1021/acs.bioconjchem.7b00569

Figure 6.

Figure 6

Antioxidant and anti-inflammatory activity of D-mino in LPS-activated BV-2 microglial cells and cytotoxicity assay. (A) Schematic image describes the drug treatment procedure. BV-2 microglia were activated with LPS at 100 ng/mL concentration before and after 3 h of drug treatment, and the culture supernatants were assayed for nitric oxide production by the Griess reaction and tumor necrosis factor α (TNF-α) release by ELISA at 24 h after the second LPS treatment. (B) Effects of free and D-mino on NO release. The concentration was indicated on the basis of free 9-amino-minocycline. (C) Effects of free and D-mino on TNF-α release. (D) BV-2 microglial viability was measured by CellTiter AQ proliferation assay. Preactivated BV-2 microglia were treated with free and D-mino for 3 h before second LPS activation. The cell viability was assayed at 24 h after the second LPS treatment. Data were expressed as mean ± standard error of the mean; the asterisk indicates p < 0.05 significant differences from the LPS alone group, and double asterisks indicate p < 0.05 significant differences between free and dendrimer conjugate.