Key resources table.
| Reagent type species |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (human) | HCN1 | Xention Ltd. (Cambridge, UK) |
NM_021072.3 | |
| Gene (mouse) | HCN2 | PMID: 11331358 | NM_008226.2 | Laboratory of Steven A. Siegelbaum |
| Gene (rabbit) | HCN4 | PMID: 10212270 | NM_001082707.1 | |
| Gene (mouse) | TRIP8b | PMID: 19555649 | Laboratory of Steven A. Siegelbaum |
|
| Strain, strain background (E. coli) |
DH5α | Thermo Fisher Scientific | ||
| Strain, strain background (E. coli) |
Stbl2 | Thermo Fisher Scientific | ||
| Strain, strain background (Mus musculus) |
Male or female C57BL/6J mice | The Jackson Laboratory | RRID:MGI:5653012 | |
| Strain, strain background (Oryctolagus cuniculus) |
New Zealand white female rabbits |
Envigo | ID strain:HsdOkd:NZW | |
| Cell line (human) | HEK 293T | ATCC | RRID:CVCL_0063 | Tested negative for mycoplasma |
| Biological sample (Mus musculus) |
Isolated adult Sinoatrial node (SAN) cardiomyocytes |
PMID: 11557233 | ||
| Biological sample (Oryctolagus cuniculus) |
Isolated adult Sinoatrial node (SAN) cardiomyocytes |
PMID: 2432247 | ||
| Recombinant DNA reagent |
pET-52b (plasmid) | EMD Millipore | ||
| Recombinant DNA reagent |
modified pET-24b (plasmid) | Laboratory of Daniel L. Minor, Jr. |
||
| Recombinant DNA reagent |
pcDNA 3.1 (plasmid) | Clontech Laboratories | ||
| Recombinant DNA reagent |
pCI (plasmid) | Promega | ||
| Recombinant DNA reagent |
TRIP8bnano (cDNA) | This paper | Made by PCR and cloning; see Constructs |
|
| Recombinant DNA reagent |
TRIP8bnano (E240R) (cDNA) | This paper | Made by site-directed mutagenesis of TRIP8bnano wt; see Constructs |
|
| Recombinant DNA reagent |
TRIP8bnano (E241R) (cDNA) | This paper | Made by site-directed mutagenesis of TRIP8bnano wt; see Constructs |
|
| Recombinant DNA reagent |
TRIP8bnano (E264K) (cDNA) | This paper | Made by site-directed mutagenesis of TRIP8bnano wt; see Constructs |
|
| Recombinant DNA reagent |
TRIP8bnano (E265K) (cDNA) | This paper | Made by site-directed mutagenesis of TRIP8bnano wt; see Constructs |
|
| Recombinant DNA reagent |
TRIP8bnano (D252N) (cDNA) | This paper | Made by site-directed mutagenesis of TRIP8bnano wt; see Constructs |
|
| Recombinant DNA reagent |
TRIP8bcore (cDNA) | PMID: 25197093 | ||
| Recombinant DNA reagent |
human HCN2 CNBD (cDNA) | PMID: 25197093 | ||
| Recombinant DNA reagent |
human HCN2 CNBD (N547D) (cDNA) |
This paper | Made by site-directed mutagenesis of human HCN2 CNBD wt; see Constructs |
|
| Recombinant DNA reagent |
human HCN2 CNBD (K665E) (cDNA) |
This paper | Made by site-directed mutagenesis of human HCN2 CNBD wt; see Constructs |
|
| Recombinant DNA reagent |
human HCN2 CNBD (K666E) (cDNA) |
This paper | Made by site-directed mutagenesis of human HCN2 CNBD wt; see Constructs |
|
| Recombinant DNA reagent |
human HCN2 CNBD (R650E) (cDNA) |
This paper | Made by site-directed mutagenesis of human HCN2 CNBD wt; see Constructs |
|
| Recombinant DNA reagent |
human HCN1 (cDNA) | Xention Ltd. (Cambridge, UK) |
||
| Recombinant DNA reagent |
TRIP8b (1a4) (cDNA) | This paper | Made by PCR and cloning; see Constructs |
|
| Recombinant DNA reagent |
mouse HCN2 (cDNA) | Laboratory of Steven A. Siegelbaum |
||
| Recombinant DNA reagent |
rabbit HCN4 (cDNA) | PMID: 10212270 | ||
| Recombinant DNA reagent |
mouse HCN2 (N520D) (cDNA) | This paper | Made by site-directed mutagenesis of mouse HCN2 wt; see Constructs |
|
| Sequence-based reagent |
human HCN1 (R549E) (cDNA) | This paper | Made by site-directed mutagenesis of human HCN1 wt; see Constructs |
|
| Peptide, recombinant protein |
TAT-TRIP8bnano (YGRKKRRQRRRGG- NHSLEEEFERAKAAVESTEFWDKMQA EWEEMARRNWISEN) |
CASLO ApS | ||
| Peptide, recombinant protein |
TAT-(SCRAMBLED)TRIP8bnano
(YGRKKRRQRRRGG-RNEAEAAE VAQKDMINERARTHEFEWESWE MWENLSESFK) |
CASLO ApS | ||
| Commercial assay or kit |
QuickChange Lightning Site- Directed Mutagenesis Kit |
Agilent | ||
| Commercial assay or kit |
Thermo Scientific TurboFect Transfection Reagent |
Thermo Fisher Scientific | ||
| Chemical compound, drug |
Adenosine 3', 5'-cyclic monophosphate (cAMP) |
SIGMA | ||
| Software, algorithm | Clampfit 10.5/10.7 | Molecular Devices | RRID:SCR_011323 | |
| Software, algorithm | CYANA-2.1 | L. A. Systems, Inc. | ||
| Software, algorithm | AMBER 12.0 |
http://pyenmr.cerm.unifi.it/
access/index/amps-nmr |
||
| Software, algorithm | HADDOCK2.2 | www.wenmr.eu |