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Treatment of EGFP-654 cells with labeled oligomers. Fluorescently labeled 2′-O-Me (TAMRA) or 2′-O-MOE (Texas Red) oligomers were used to treat EGFP-654 cells either by scrape loading (1 µM) or by cationic lipid delivery (0.03 µM). Images were generated by sequential excitement of the oligomer label followed by EGFP. EGFP (green) and oligomer (red) images were merged, with co-localization appearing as shades of yellow.
