Fig. 3.

Rap1 activity is required for EphB4-Fc- and efnB2/CTF2-induced sprouting. a BAMECs were grown on collagen-coated Cytodex® microcarrier beads and transferred to fibrinogen solution. Pre-clustered Fc or EphB4-Fc (4 μg/ml) was added to the fibrinogen solution as well as DMSO or GGTi-298 (0.5 μM) then solidified with thrombin to form fibrin gels. (Left) Phase-contrast images show BAMEC cells sprouting from a microcarrier bead. (Right) The percentage of sprouts exceeding the diameter of the bead relative to control (Fc) was determined. Paired t test (n = 3, *p < 0.05, error bars = SEM). b Cells were transduced with pMX or pMX expressing efnB2/CTF2-myc³. Cells were grown on collagen-coated Cytodex® microcarrier beads and allowed to sprout in the presence of DMSO (vehicle) or GGTi-298 (0.5 μM). (Left) Phase-contrast image of BAMEC cells sprouting from a microcarrier bead. (Right) The percentage of sprouts exceeding the diameter of the bead relative to control (pMX) was determined. Unpaired t test (*p < 0.05, error bars = SEM) and paired t test (*p < 0.05, error bars = SEM) n = 4