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. Author manuscript; available in PMC: 2019 Jun 27.
Published in final edited form as: Cell Syst. 2018 May 30;6(6):655–663.e5. doi: 10.1016/j.cels.2018.05.002

Figure 2. Developing an optogenetic system for spatial control over liquid droplet disassembly.

Figure 2

(a) Constructs used to create the PixELL optogenetic system and (b) schematic of blue light-dissociable intracellular droplets. (c) Representative images of intracellular clusters before and after 450 nm light-induced dissociation. (d) Quantification of photoswitchable clustering during 5 cycles of dissociation and aggregation. Mean ± SEM are shown for 8 representative cells. Images from c are shown as insets to relate intracellular droplet patterns to SNR quantification. (e) Visualization of two PixELL droplet fusion events. (f) Droplet intensity during FRAP experiments indicating photobleaching at t=0 and recovery over 10 min. Mean ± SEM are shown for 5 cells, normalized to initial intensity. See also Figures S2–S3 and Movies S4–S5.