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. Author manuscript; available in PMC: 2019 Jun 27.
Published in final edited form as: Cell Syst. 2018 May 30;6(6):655–663.e5. doi: 10.1016/j.cels.2018.05.002

Figure 4. PixELLs amplify shallow stimulus gradients into all-or-none spatial patterns of droplets.

Figure 4

(a) Gradient stimulation of a PixELL-expressing NIH3T3 cell. Fluorescent images of FUSN-FusionRed-PixD are shown for a representative cell stimulated with a linear gradient of light intensity. (b) Kymograph of maximum FUSN-FusionRed-PixD fluorescence within each row of the yellow box from a (right), and median blue light intensity measured within the yellow box from a (left). (c) Quantification of the kymograph in b at 35 min, after spatial light pattern is established. A gradual decrease in 450 nm intensity (top panel; blue curve) elicits a sharp, switch-like transition to form bright FUSN-FR-PixD droplets (bottom panel; red curve). See also Movie S7.