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. Author manuscript; available in PMC: 2019 Jun 27.
Published in final edited form as: Cell Syst. 2018 May 30;6(6):655–663.e5. doi: 10.1016/j.cels.2018.05.002

Figure 5. Membrane-localized optoDroplets retain spatial memory of transient stimuli.

Figure 5

(a) Schematic of Myr-optoDroplet construct and mode of activation. (b) Still images of Myr-FUSN-FusionRed-Cry2 for a cell exposed to a transient, local 450 nm stimulus. (c) Quantification of total intensity for membrane regions inside and outside the stimulus mask, respectively. Mean ± SEM are shown for 3 cells. (d) Schematic and still images of Myr-FUSN-FusionRed-Cry2 localization in the membrane plane for a cell exposed to a local 450 nm stimulus (dashed blue box) followed by global 450 nm illumination. (e) Quantification of total intensity in membrane regions inside and outside the stimulus mask, respectively. Mean ± SEM are shown for 3 cells. See also Movies S8–S9.