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. Author manuscript; available in PMC: 2019 Jun 27.
Published in final edited form as: Cell Syst. 2018 May 30;6(6):655–663.e5. doi: 10.1016/j.cels.2018.05.002

Figure 6. Liquid phase separation drives spatial memory in RTK signaling.

Figure 6

(a) Schematic showing FGFR1-optoDroplets for inducing RTK clustering and downstream signaling. (b) FGFR1-optoDroplet cells reversibly “cringe” in response to global blue light stimulation. (c) Quantification of change in cell surface area for cell pictured in b. (d) FGFR1-optoDroplet cells retract in response to light, ‘avoiding’ a local light stimulus. (e) FGFR1-optoDroplet cells exhibit persistent local clustering and cytoskeletal contraction even after a switch to global illumination. (f) Quantification of cell surface area within the local-to-global illuminated region (blue box in e) and global-only illuminated region (remainder of cell in e) during local-to-global illumination. See also Figure S5 and Movies S10–S12.