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. Author manuscript; available in PMC: 2018 Nov 28.
Published in final edited form as: Nat Ecol Evol. 2018 May 28;2(7):1155–1160. doi: 10.1038/s41559-018-0569-4

Figure 3. Eye phenotype and associated gene expression changes in wild type and DNA methylation-deficient Danio rerio.

Figure 3

a-b, Transmitted light photomicrographs of the heads of 48 hpf wild type sibling (a) and tet2-/-, tet3-/- double mutant (b) embryos. c, Quantitation of eye size in 48 hpf wild type sibling and tet2-/-, tet3-/- double mutant embryos. Histograms show mean, error bars represent s.d. in the relative eye size (n =10), two-tailed t-test, *p<0.05. d-g, Assessment of crx (d,f) and gnb3a (e,g), promoter DNA methylation in isolated eyes from 48 hpf wild type sibling (d,e) and tet2-/-, tet3-/- double mutant (f,g) animals. h, Quantitative RT-PCR analysis of the percent relative expression of crx and gnb3a in isolated eyes from 48 hpf wild type sibling (orange columns) and tet2-/-, tet3-/- double mutant (blue columns) animals. Histograms show mean, error bars represent s.e.m of three biological replicates; two-tailed t-test, *p<0.005. Scale bar 100μM in b for a and b.