Figure 6.
miR-3178 Inhibits Cancer Cell Metastasis In Vivo
(A–E) Metastasis detection by fluorescence signals. 1E8 cells stably expressing miR-3178 and dsRed fluorescent protein were injected into BALB/c nude mice (n = 9) through tail vein (2 × 106 per inoculation). Mice were sacrificed after 7 weeks, and fluorescence signals of whole body (A) and specific organs (B, lung; C, lymph node; D, liver) were detected and analyzed (E). (F) H&E staining is shown. Arrowheads indicate cancer metastasis foci. The scale bar represents 4×, 2 mm; 10×, 500 μm; and 40×, 200 μm. (G) 1E8 cells were co-transfected with miR-3178 and pCNDA3.1-TRIOBP-1 (TRIOBP-1) or control vector (Ctrl) for 24 hr, followed by western blotting and Transwell assays. *p < 0.05 and **p < 0.01.