Figure 3.

Cisterna Magna Delivery of scAAV9_hSOD1si in SOD1^G93A Mice at Post-natal Day 1 Efficiently Improves Lumbar Motor Neuron Survival at End Stage and Reduces Gliosis

(A and B) Representative images of motor neurons in lumbar spinal cord ventral horn from mice treated at P1 with scAAV9_hSOD1si (A, main 20×, inset 63×; scale bar, 100 μm) or scAAV9_hSOD1ssi (B, main 20×, inset 63×; scale bar, 100 μm), stained for CGRP (green), and quantified in (C) (n = 20 sections/mouse, 5 mice per group). (C–H) Representative images of Nissl-stained lumbar spinal cord from mouse treated at P1 with scAAV9_hSOD1si (D, scale bar, 100 μm; E, scale bar, 250 μm) or scAAV9_hSOD1ssi (F, scale bar, 100 μm; G, scale bar, 250 μm) and quantified in (H) (n = 10 sections/mouse, 3 mice per treatment group). (I–L) Representative images of lumbar cord ventral horn from mouse treated at P1 with scAAV9_hSOD1si (I,scale bar, 50 μm; J, scale bar, 100 μm) or scAAV9_hSOD1ssi (K, scale bar, 50 μm; L, scale bar, 100 μm) and stained for GFAP (magenta), Iba1 (yellow), and DAPI (cyan). *p < 0.05.