Figure 3.

Evaluation of the Targeting of Mammalian Cells by the Engineered Bacteriophage Nanocarrier

(A) Immunofluorescence-based bacteriophage binding assay. Cultured HEK293T cells were incubated with the RGD- or NS-bacteriophage. The red color represents fluorescence from bacteriophage staining, and the blue color shows fluorescence of DAPI-stained cell nuclei. The scale bars represent 100 μm. (B) GFP expression observed after transfection of HEK293T cells with the RGD- or NS-bacteriophage is shown. The scale bars represent 100 μm. (C) Quantitative analysis of GFP level in the presence or absence of fibronectin is shown. Experiments were performed in triplicate and data presented as percentage of the mean of relative fluorescence units (RFU) of treated cells compared with the control HEK293T cells stably expressing GFP. Significant difference: n.s., not significant, ***p < 0.001