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. 2018 May 3;12:57–66. doi: 10.1016/j.omtn.2018.04.016

Figure 4.

Figure 4

miR-125b Promotes the Contraction of JS1 Cells

(A) Inhibition of miR-125b attenuated the TGF-β-induced contraction of JS1 cells. The collagen lattice containing JS1 cells that were transfected with the indicated RNA was incubated without (−) or with (+) 5 ng/mL TGF-β for 24 hr, then released, followed by culture for another 24 hr before calculating contraction areas. (B) Antagonism of miR-125b inhibited the contraction of JS1 cells. (C) Ectopic expression of miR-125b promoted the contraction of JS1 cells. For (B) and (C), the collagen lattice containing JS1 cells that were transfected with miR-125b inhibitor (50 nM) or miR-125b mimic was incubated with 10% FBS-containing DMEM for 24 hr; the gels were then released, followed by culture for another 48 (B) or 24 (C) hr before measuring contraction areas. Data are presented as mean ± SEM in (A)–(C). *p < 0.05; **p < 0.01.