Fig. 3.

Effect of homocysteine on M. smegmatis pellicle and biofilm formation. a M. smegmatis cells were grown in liquid media with increasing homocysteine concentration in the absence of Tween-80 at static condition. The images showed decreased pellicle formation on addition of 0.4 mM homocysteine. b M. smegmatis cells were grown in minimal media under non-shaking conditions and in the absence of Tween-80 to develop biofilm. The images show decrease in biofilm as concentration of homocysteine (Hcy) is increased. Biofilm formation was completely absent after 0.4 mM homocysteine