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. 2018 May 23;596(13):2491–2506. doi: 10.1113/JP274731

Figure 4. Ca2+ signalling in SPV SMCs induced by ATP.

Figure 4

A, fluorescence confocal image of a SPV loaded with the intracellular Ca2+ indicator Oregon green‐BAPTA‐1/AM showing VSMCs and ECs lining the SPV lumen. B, simultaneous changes in [Ca2+]i in a VSMC (top trace) and SPV contraction (bottom trace) during stimulation with ATP (50 μm). The changes in [Ca2+]i are expressed as the ratio (F/F 0) of fluorescence measured in a small square region (49 μm2) within a VSMC (white square in A). SPV contraction is expressed as the change in lumen area of the imaged SPV region. The experiment is representative of 12 experiments from different slices from four rats. C, line‐scan plot from a region across the wall of the SPV (indicated by dotted line a in A) showing ATP‐induced Ca2+ oscillations (vertical white lines) in two VSMC. D, line‐scan plot from a region along the longitudinal axis of a single VSMC (indicated by dotted line b in A) showing the Ca2+ wave propagation associated with the Ca2+ oscillations (vertical white lines).