Figure 5.

The Effect of α-Amanitin on Transcription from Pol III Promoters

(A) Pol II/III promoters were cloned into the P-T6-pA-Luc backbone. (B) The P-T6-pA-Luc constructs were individually transfected into HEK293T cells with or without α-amanitin (2 μg/mL) treatment. RNA extraction and northern blotting were performed as in Figure 4C. The Pol III/II transcripts are indicated. The ** signals reflect minor Pol III transcripts (see legend to Figure 4C). The results were reproduced in a second independent experiment. (C) The P-T6-Luc constructs were individually transfected into HEK293T cells with or without α-amanitin (2 μg/mL). RNA extraction and northern blotting were performed as in Figure 4D.