Figure 6.

Inactivation of the TATA Box of Type 3 Pol III Promoters Abolishes Pol III Transcription, but It Stimulates Pol II Activity

(A) Mutation of the TATA boxes of the three Pol III promoters was achieved by changing the TATA sequence (+) to TCGA (−). (B) Northern blot analysis was performed as described in Figure 4C. The pBS construct was used as a negative control. The ∼44-nt transcripts (black triangle) represent Pol III transcripts terminated at T6. (C) The Luc activity of the different promoter constructs with (+) or without (−) TATA box. The pBS served as a negative control. The data are shown as mean values ± SD (n = 3).