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. 2018 May 29;12:174–183. doi: 10.1016/j.omtn.2018.05.004

Figure 2.

Figure 2

Expressions of uc.454 Affected Cell Proliferation and Apoptosis in Lung Cancer Cell Lines

(A) Ectopic expression of uc.454 was confirmed by real-time qPCR after transfection of NCI-H2170 and SPC-A-1 cells. (B) CCK-8 assays were used to assess cell proliferations in different LC cell lines and groups after transfection. (C and D) Colony formation assays were performed to determine the proliferation of NCI-H2170 and SPC-A-1 cells after transfection. (E and F) Flow cytometric analysis showed that uc.454 induces cell apoptosis in NCI-H2170 and SPC-A-1 cells. Error bars represent mean ± SD of triplicate experiments. *p < 0.05.