Figure 3.

Overexpression of uc.454 Inhibited Tumor Growth In Vivo

(A) NCI-H2170 LC cells (2 × 10⁶) stably overexpressing uc.454 or negative control were inoculated into nude mice. Four weeks later, the primary tumors were obtained. (B) Tumor volumes of uc.454-shRNA and negative control were compared. (C) Kaplan-Meier analysis of overall survival of mice were recorded and compared (n = 20 mice per group). (D) HSPA12B expression was examined by IHC staining in primary tumor specimens in two groups. The tumors developed from the uc.454 cells displayed weaker HSPA12B staining than the tumors formed by the control group cells in the immunohistochemistry (IHC) analysis. Error bars represent mean ± SD of triplicate experiments. *p < 0.05.